In order to understand the influence of annealing temperature on PbSe quantum dots doped glass produced by a melt-annealing technique, experiments are carried out to compare the influences of different nucleation durations, crystallization temperatures and time on the particle size, distribution and absorption spectrum. Under the condition of the same nucleation temperatures and different crystallization temperatures, the transmission electron microscope images of all samples show that a certain quantity of PbSe crystals are crystallized in the glass. While the particle sizes and densities are slightly different. The calculated distribution of the particle sizes quantitatively indicates that the particle size will be enlarged with the increase of crystallization temperature and the crystal particle density. The measured absorption spectrum shows that the peak value of absorption spectrum increases gradually with increasing the crystallization temperature. At the same time, the peak value shows a red-shift phenomenon. While under the relatively low crystallization temperature, the infrared absorption peak cannot be obtained in spite that some crystals have grown inside the glass. The absorption spectrum is covered up by the background signals because of the relatively smaller particle size and density. This work will be benefit of producing different size quantum dots with a certain density, and realizing stronger absorption and emission in multiband.